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Gibco™ Kit del sistema de expresión ExpiCHO™
Descripción
Includes
2 viales de células ExpiCHO-S™ congeladas, 1 frasco de 1000 ml de medio de expresión ExpiCHO™, 1 kit de transfección ExpiFectamine™ CHO para 1 l de cultivo, 1 vial de anticuerpo que expresa el vector de control positivo, 1 frasco de 100 ml OptiPRO™ SFM; almacene todos los componentes a entre +2 °C y -8 °C, excepto las células congeladas, que deben almacenarse en nitrógeno líquido
The ExpiCHO Expression System Kit combines the power of rapid, ultra–high-yield transient protein production in suspension culture with the benefit of expression in Chinese hamster ovary (CHO) cells. It is based on high-density culture of ExpiCHO-S cells in ExpiCHO Expression Medium. Transient expression is powered by ExpiFectamine CHO transfection reagent, designed specifically for high-density CHO suspension culture, in combination with a specialized transfection enhancer and culture feed.
All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.
The ExpiCHO Expression System enables:
- Milligram to multigram yields of recombinant protein per liter of culture in 5–14 days post-transfection
- Robust and reproducible transfection results, for greater confidence in results and ability to use CHO cells from research to discovery
- Transfection efficiency of > 90% for high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
- Use of one-third to one-half less plasmid DNA than other transfection reagents
- Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows
- Scalable expression for culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks
The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO SFM, and an antibody-expressing positive control vector.
ExpiCHO-S cells, ExpiCHO Expression Medium, ExpiFectamine CHO Transfection Kit, and OptiPRO SFM may also be purchased separately.
All components of the system are animal origin–free.
Especificaciones
Especificaciones
| Tipo | Sistema de expresión |
| Etiqueta de proteína | Sin marcar |
| Tipo de entrega | Transfección |
| Cantidad | 1 kit |
| Contenido y almacenamiento | • 2 viales de células ExpiCHO-S congeladas • 1000 ml de medio de expresión ExpiCHO • 1 kit de trasnfección ExpiFectamine CHO para 1 l de cultivo • 1 vial de anticuerpo que expresa el vector de control positivo • 100 ml de OptiPRO SFM Almacenar todos los componentes a 2-8 °C, excepto las células congeladas, que deben almacenarse en nitrógeno líquido. |
| Condiciones de envío | Aprobado para su envío a temperatura ambiente o en hielo húmedo o seco |
| Línea de células | CHO-S, adaptada SFM |
| Sistema de expresión | Mamífero |
| Línea de productos | ExpiCHO™ |
| Para utilizar con (aplicación) | Producción de proteínas, expresión constitutiva |
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