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Gibco™ Advanced DMEM/F-12
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.
Marca: Gibco™ 12634010
60.20 CHF valido fino al 2025-03-28
Usa il codice promo "24107" per avere il tuo prezzo promozionale.
53.50 CHF valido fino al 2025-06-21
Usa il codice promo "24112" per avere il tuo prezzo promozionale.
Allerta:
To receive the discount customers must purchase three of the same product at list price in a single order to receive 33.33% discount. There is no limit to the multiples of 3 that customers can buy. Use promo code ”24112” to get your promotional price
Requires : Supplementation
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Descrizione
Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.
This Advanced DMEM/F-12 is manufactured as follows:
With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine, HEPES
The complete formulation is available.
Using Advanced DMEM/F-12
Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Specifica
| MRC-5, SP2, Vero, WI-38 and Jurkat | |
| 1 X | |
| DMEM (Dulbecco's Modified Eagle Medium)/F-12 | |
| Sterile-filtered | |
| High Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA) | |
| cGMP-compliant under the ISO 13485 standard | |
| 500 mL | |
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Animal Origin | |
| Liquid | |
| Reduced Serum | |
| Sterile-filtered | |
| No Glutamine, No HEPES | |
| Gibco™ | |
| Room Temperature |
Sicurezza e movimentazione
Identificativo prodotto
Avvertenza
Categoria di pericolo
Indicazioni di pericolo
Consigli di prudenza
Informazioni supplementari
- Methyl benzoate
Avvertenza
- Attenzione
Categoria di pericolo
- Tossicità acuta Categoria 4
Indicazioni di pericolo
- H302-Nocivo se ingerito.
Consigli di prudenza
- P301+P312-IN CASO DI INGESTIONE accompagnata da malessere: contattare un CENTRO ANTIVELENI/medico/
Informazioni supplementari
- MIXTURE LIST-Contiene: Methyl benzoate
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Titolo del prodotto
For Research Use Only. Not for use in diagnostic procedures.
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