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Cytiva HiScreen™ Capto™ Phenyl (High Sub) Prepacked HIC Column

Product Code. 11648943 Shop All Cytiva Products
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Quantity:
1 x 4.7mL
Unit Size:
Each
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Hydrophobic interaction chromatography (HIC) media used in the capture and intermediate stages of protein purification, used for method optimization and parameter screening

  • Highly cross-linked agarose matrix with average bead size of 75μm
  • Has good flow properties and high loading capacities
  • Bed height of 10cm designed to allow method optimization and parameter screening
  • Connect easily in series to achieve 20cm bed height
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Capto Phenyl (high sub) media is used for initial and intermediate step purifications requiring a chromatography medium with medium to high hydrophobicity
  • Combines high capacity with high flow velocity and low backpressure to reduce process cycle times and increase productivity
  • Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

  • Bed volume: 4.7mL
  • Bed dimension: 7.7 × 100mm
  • Flow rate: 1.2 to 2.7mL/min.
  • Flow velocity: <600cm/hr.
Media
  • Average particle size: 75μm
  • Degree of substitution: 27μmol/mL gel
  • pH stability cleaning: 2 to 14
  • Pressure/Flow specification: 300kPa at 600cm/hr., 1m diameter column, 20cm bed height
  • Medium: BioProcess
  • pH stability working range: 3 to 13
  • Ligand: phenyl
  • Binding capacity/mL chromatography medium: 27mg BSA /mL (at QB10%)
Column
  • Bed support: filter
  • Material (column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm

TRUSTED_SUSTAINABILITY

Specifications

Buffer Aqueous Buffer
Product Type HiScreen Capto Phenyl (high sub) column
Bed Volume 4.7mL
Diameter (English) Inner 0.3 in.
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Height (English) 3.93 in.
Height (Metric) 100mm
Flow Rate <600cm/hr.
Material Polypropylene
Ligand Type Phenyl (∼27μM/mL drained medium)
Max. Pressure 43.5psi
pH Range 3 to 13
Particle Size 75μm
Support Type Highly Cross-linked Agarose
Content And Storage 4° to 30°C
Column Type HIC Column
For Use With (Application) Optimization and parameter screening
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Safety and Handling

compliance-icons
Product Identifier
  • HiScreen Capto Phenyl (high sub), 1 x 4.7 ml
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
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