Invitrogen™ Kit de ELISA para IGFBP1 humano

Descripción

The Human Insulin-like Growth Factor Binding Protein 1 (Hu IGFBP1) ELISA quantitates Hu IGFBP1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IGFBP1. Principle of the method The Human IGFBP1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Este gen es miembro de la familia de proteínas de unión al factor de crecimiento similar a la insulina (IGFBP) y codifica una proteína con un dominio IGFBP y un dominio de tiroglobulina tipo I. La proteína se une a los factores de crecimiento similares a la insulina (IGF) I y II y circula en el plasma. La unión de esta proteína prolonga la semivida de los IGF y altera su interacción con los receptores de la superficie celular.