Invitrogen™ Kit de ELISA para MMP1 humano

Descripción

The Human Matrix MetalloProteinase 1 (Hu MMP1) ELISA quantitates Hu MMP1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu MMP1. Principle of the method The Human MMP1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La metaloproteinasa de matriz (MMP) son enzimas proteolíticas capaces de degradar los componentes del tejido conectivo. La secreción desequilibrada de ciertas MMP o las alteraciones en el control diferencial de las MMP por el inhibidor tisular de las MMP (TIMP) están implicadas en el potencial invasivo de los tumores malignos. La colagenasa intersticial (MMP-1), la gelatinasa de 72 kDa A/colagenasa de tipo IV (MMP-2) y gelatinasa de 92 kDa B/colagenasa de tipo IV (MMP-9) disuelven la matriz extracelular (ECM) y pueden iniciar y promover la angiogénesis.