Invitrogen™ Kit de ELISA para peroxidasa tiroidea humana

Descripción

The Human Thyroid Peroxidase ELISA quantitates Hu Thyroid Peroxidase in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Thyroid Peroxidase. Principle of the method The Human Thyroid Peroxidase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Este gen codifica una glicoproteína unida a la membrana. La proteína codificada actúa como una enzima y desempeña un papel central en la función de la glándula tiroides. La proteína funciona en la yodación de residuos de tirosina en la formación de tiroglobulina y fenoxi-éster entre pares de tirosina yodada para generar las hormonas tiroideas, tiroxina y triyodotironina. Las mutaciones en este gen están asociadas con varios trastornos de la hormonogénesis tiroidea, incluyendo hipotiroidismo congénito, bocio congénito y defecto de organificación de la hormona tiroidea IIA. Para este gen se han identificado múltiples variantes de transcripción que codifican isoformas distintas. Se han descrito variantes de empalme adicionales, pero no se han determinado sus naturalezas biológicas.