Invitrogen™ Kit de ELISA para IGFBP-3 de ratón

Descripción

The Mouse IGF Binding Protein 3 (Ms IGFBP-3) ELISA quantitates Ms IGFBP-3 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms IGFBP-3. Principle of the method The Mouse IGFBP-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La proteína de unión al factor de crecimiento similar a la insulina 3 (IGFBP3) forma parte de la superfamilia de proteínas de unión al factor de crecimiento similar a la insulina (IGF) que incluye seis proteínas de unión a IGF de alta afinidad (IGFBP) y al menos cuatro proteínas de unión de baja afinidad, denominadas proteínas relacionadas con IGFBP (IGFBPrP). Los miembros de la IGFBP son proteínas ricas en cisteína con residuos de cisteína conservados agrupados en las regiones aminoterminal y carboxi-terminal de la molécula. La secuencia de ADNc que codifica la IGFBP3 humana madura se fusiona al péptido de señal de CD33. La IGFBP3 humana madura recombinante del residuo aminoácido 265 tiene una masa molecular calculada de aproximadamente 29 kDa. Debido a la glicosilación, la proteína recombinante migra como 41 kDa en SDS-PAGE.