Thermo Scientific™ Verso 1-step RT-qPCR Kit, SYBR Green, ROX

Descripción

The Thermo Scientific Verso 1-step RT-qPCR ROX Kit for SYBR Green chemistry offers a quick and simple way to detect mRNA.

An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment. All Verso 1-step RT-qPCR kits incorporate an inert dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

In a 1-step approach to RT-qPCR, both the RT step and qPCR steps are performed in a single tube (1-step RT-qPCR). Adopting a 1-step approach is ideal for primer sequence optimization and collecting comparative data in experiments that do not require subsequent cDNA storage. The approach minimizes the risk of contamination, and improvements in reproducibility may be observed as a result of reduced sample handling.

Verso One-Step RT-qPCR Kits for SYBR Green chemistry are compatible with all major real-time thermal cyclers.

• Elimine la necesidad de tratamientos con ADNasa: RT Enhancer (opcional) previene el arrastre del ADNg
• Pasos de RT y qPCR realizados en un solo tubo: comodidad y reducción del riesgo de contaminación
• Uso de la reacción RT completa como plantilla para qPCR: sensibilidad mejorada
• La enzima Verso RT es muy sensible con un amplio rango dinámico: detección precisa de ARN de 1 pg a 1 μg.
• La enzima Verso RT tiene un amplio intervalo de temperaturas de trabajo (de 42 a 57 °C): éxito mejorado con las plantillas enriquecidas en GC y otras plantillas difíciles
• Contiene un colorante azul inerte para facilitar el pipeteo durante la configuración de la reacción